Equipment

 On the basis of the Biotechnological Business-Incubator of MSU there is placed modern equipment complying with the high world level of scientific research.

 

Histology and immunohistochemistry

 

The automated tissue processor Leica TP1020,
Automatic device for staining tissue sections Leica ST5010 Autostainer XL

 

 

 

Preparation of histological specimens

Pathological examination

Cryostat Leica CM1950

 

 

Preparation of cryosections for specific staining techniques (immunohistochemistry, coloration on lipids, etc.)

 

Biochemistry and Haematology 

 

Spectrophotometer BUsing AU480

 

 

 

Biochemical analysis of serum and urine: aspartate aminotransferase and alanine, alpha-amylase, total and direct bilirubin, alkaline phosphatase, lipase, calcium, creatinine, total protein, urea

Hematology Analyzer BUsing LH 750

 

 

Hematology whole blood: red cells, platelets, lymphocytes, leucocytes, hematocrit

 

Flow cytometry and sorting

 

 

The flow cytometer-sorter BC Navios

 

The flow cytometer-sorter BD FACSAria III

Identification of individual cell classes, subclasses, populations, sub-populations, measuring their surface or cytoplasmic markers

Evaluation of the functional state of the cells (mitochondrial potential, intracellular calcium, pH) with special ratio-metric dyes (FDA, DiOC1, JC -1, etc.)

Evaluation of the functional state of cells using specific antibodies to the phosphorylated and dephosphorylated forms of the proteins under study

Gene expression and transfection efficiencies - GFP-structure
Analysis of the cell cycle and DNA content in live and fixed cells

The study of the kinetic parameters of cellular processes (cell membrane damage, enzyme activity)

Investigation of the mechanisms and stages of apoptosis using a variety of markers and probes

Study of phagocytosis, including blood neutrophils and macrophages

Investigation of the sensitivity of cells to cytostatics by determining the expression level of proteins (P-gp, etc.).

Molecular genetic studies (the study of individual chromosomes and gene expression, ploidy, determination of the size of the DNA fragments)

Flowing karyotyping chromosomes with fluorescent probes

Sort cells and particles up to 4 fractions simultaneously. It is also possible for sorting slides, 6, 24, 48, 96, 384 plantshety well.

 

Molecular biology

 

Thermocycler Applied Biosystems GeneAmp PCR System 9700

 

The system of real-time PCR QuantStudio 7

 

 

Primer selection system

Carrying out of sample preparation for polymerase chain reaction (PCR)

Analysis of DNA and RNA by PCR and RT-PCR (RT-PCR)

Setting Real-Time PCR

Analysis of gene expression

Analysis of telomerase activity and gene expression of telomerase complex.

Analysis of real-time length telomer.v QuantStudio 7